PubReading [99] - MicroED: conception, practice and future opportunities - M. Clabbers, A. Shiriaevaa and T. Gonen

<p>This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The <strong>cryo-EM method</strong> <strong>microcrystal</strong> electron diffraction is described and put in the context of macromolecular electron <strong>crystallography</strong> from its origins in 2D crystals of membrane proteins to today’s application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future. - doi.org/10.1107/S2052252521013063 - 2022</p>