PubReading [246] - Mechanistic insights into mRNA 3'-end processing - A. Kumar, M. Jinek et al.

<p>The <strong>polyadenosine (poly(A))</strong> tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. <strong>mRNA</strong> 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A) tail, and trigger transcription termination. The polyadenylation site (PAS) defines the end of the 30-untranslated region (3'-UTR) and, therefore, selection of the cleavage site is a critical event in regulating <strong>gene expression</strong>. Integrated structural biology approaches including biochemical reconstitution of multi-subunit complexes, cross-linking mass spectrometry, and structural analyses by X- ray crystallography and single-particle electron cryo-microscopy (cryoEM) have enabled recent progress in understanding the molecular mechanisms of the mRNA 3'-end processing machinery. Here, we describe new molecular insights into pre-mRNA recognition, cleavage and polyadenylation.</p><p><a href="https://doi.org/10.1016/j.sbi.2019.08.001"><em>https://doi.org/10.1016/j.sbi.2019.08.001</em></a><em> - 2019</em></p>