PubReading [201] - The cryo-EM method microcrystal electron diffraction (MicroED) - B. Nannenga and T. Gone

<p>In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (<strong>MicroED</strong>). Since that time, <strong>data collection</strong> and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example <strong>structures</strong> solved by MicroED and provide practical advice to prospective users.</p><p>doi.org/10.1038/s41592-019-0395-x - 2019</p>