PubReading [129] - Beginner’s guide to mass spectrometry–based proteomics - A. Sinha and M. Mann

<p><strong>Mass spectrometry</strong> (MS)-based proteomics is the most comprehensive approach for the quantitative profiling of proteins, their interactions and modifications. It is a challenging topic as a firm grasp requires expertise in biochemistry for sample preparation, analytical chemistry for instrumentation and computational biology for data analysis. In this short guide, we highlight the various components of a mass spectrometer, the sample preparation process for conversion of <strong>proteins</strong> into peptides, and quantification and analysis strategies. The advancing technology of MS-based proteomics now opens up opportunities in clinical applications and <strong>single-cell analysis</strong>. - <a href="https://doi.org/10.1042/BIO20200057">doi.org/10.1042/BIO20200057</a> - 2020</p>