Anchor-Based Bisulfite Sequencing (Ben Delatte)

<p>In this episode of the Epigenetics Podcast, we caught up with Ben Delatte Research Scientist at Active Motif to talk about his work on Anchor Based Bisulfite Sequencing.</p> <p>Whole Genome Bisulfite Sequencing (WGBS) is the current standard for DNA methylation profiling. However, this approach is costly as it requires sequencing coverage over the entire genome. Here we introduce Anchor-Based Bisulfite Sequencing (ABBS). ABBS captures accurate DNA methylation information in Escherichia coli and mammals, while requiring up to 10 times fewer sequencing reads than WGBS. ABBS interrogates the entire genome and is not restricted to the CpG islands assayed by methods like Reduced Representation Bisulfite Sequencing (RRBS). The ABBS protocol is simple and can be performed in a single day.</p> <p class="md-end-block md-p"> </p> <p class="md-end-block md-p">References</p> <ul class="ul-list"><li class="md-list-item"> <p class="md-end-block md-p">Chapin, N., Fernandez, J., Poole, J. et al. Anchor-based bisulfite sequencing determines genome-wide DNA methylation. Commun Biol 5, 596 (2022). <a href='https://doi.org/10.1038/s42003-022-03543-1'>https://doi.org/10.1038/s42003-022-03543-1</a></p> <p class="md-end-block md-p"> </p> </li> </ul> <p class="md-end-block md-p">Related Episodes</p> <ul class="ul-list"><li class="md-list-item"> <p class="md-end-block md-p"><a href='https://www.activemotif.com/podcasts#katja-kobow'>The Role of DNA Methylation in Epilepsy (Katja Kobow)</a></p> </li> <li class="md-list-item"> <p class="md-end-block md-p"><a href='https://www.activemotif.com/podcasts#deborah-bourchis'>DNA Methylation and Mammalian Development (Déborah Bourc'his)</a></p> </li> <li class="md-list-item"> <p class="md-end-block md-p"><a href='https://www.activemotif.com/podcasts#charlotte-ling'>Effects of DNA Methylation on Diabetes (Charlotte Ling)</a></p> </li> </ul> <p class="md-end-block md-p"> </p> <p class="md-end-block md-p">Contact</p> <ul class="ul-list"><li class="md-list-item"> <p class="md-end-block md-p"><a hr