#44 DNA tagging and Porcupine with Kathryn Doroschak

<p>Porcupine is a molecular tagging system—a way to tag physical objects with pieces of DNA called <em>molecular bits</em>, or <em>molbits</em> for short. These DNA tags then can be rapidly sequenced on an Oxford Nanopore MinION device without any need for library preparation.</p> <p>In this episode, <a href="http://katie.doroschak.com">Katie Doroschak</a> explains how Porcupine works—how molbits are designed and prepared, and how they are directly recognized by the software without an intermediate basecalling step.</p> <p>Links:</p> <ul> <li><a href="https://www.biorxiv.org/content/10.1101/2020.03.06.981514v1">Porcupine: Rapid and robust tagging of physical objects using nanopore-orthogonal DNA strands</a> (Kathryn Doroschak, Karen Zhang, Melissa Queen, Aishwarya Mandyam, Karin Strauss, Luis Ceze, Jeff Nivala)</li> </ul>