131: Virus Detection in Grapevines

<p>With the prevalence of Leaf Roll Three, Red Blotch, and other viruses, accurate and timely detection of viruses in grapevines has never been more imperative. Alan Wei, Owner and Lab Manager at Agri-Analysis LLC in Davis California explains how his lab is using next generation sequencing (NGS) to find new viruses. Currently, polymerase chain reaction (PCR) is the widely accepted method for testing for viruses. This process tests for one gene at time. Next generation sequencing allows labs to test multiple genes at a time and get results much faster.</p> <h2>References:</h2> <ul> <li><a href= "https://www.vineyardteam.org/resources/resource-library/pest-management.php?id=617"> 20: Dr. Mark Fuchs | Red Blotch Virus in Grapevines</a></li> <li><a href= "https://www.vineyardteam.org/resources/resource-library/product-assurance-and-business-sustainability.php?id=742"> 49: Stopping the Spread of Red Leaf Viruses</a></li> <li><a href= "https://www.vineyardteam.org/resources/resource-library/pest-management.php?id=793"> 71: New Techniques to Detect Grapevine Leafroll Disease</a></li> <li><a href="http://www.agri-analysis.com/">Agri-analysis LLC</a></li> <li><a href="https://www.vineyardteam.org/scholarship/">Donate: Juan Nevarez Memorial Scholarship</a></li> <li><a href="https://fps.ucdavis.edu/fgrmain.cfm">Grape Program at Foundation Plant Services</a></li> <li><a href= "https://s3-us-west-2.amazonaws.com/sites.cahnrs.wsu.edu/wp-content/uploads/sites/66/2010/07/virus-ext-bull.pdf"> Leafroll 3 Virus (GLRaV3) AKA Grapevine Leafroll Disease in Washington</a></li> <li><a href= "https://www.thermofisher.com/us/en/home/life-science/sequencing/sequencing-learning-center/next-generation-sequencing-information/ngs-basics/what-is-next-generation-sequencing.html"> Next Generation Sequencing (Deep Sequencing)</a></li> <li><a href= "https://www.genome.gov/about-genomics/fact-sheets/Polymerase-Chain-Reaction-Fact-Sheet"> PCR (Polymerase Chain Reaction) Testing</a></li> <li><a href= "https://owri.oregonstate.edu/sites/agscid7/files/r